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| The GENOMED Silica Membranes are non-charged materials that bind nucleic acids within defined parameters. Over the last years, Genomed has developed a number of different silica membranes, which solve individual problems of DNA binding and purification. The silica membranes were designed for use in spin cups or columns and microplates for high-throughput procedures. |
| Silica membrane specifications: | |
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Silica based materials |
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Individually modified membrane surfaces |
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Selective adsorption of DNA |
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Always following the "bind-wash-elute" principle |
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DNA binding under high salt conditions |
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DNA elution under low salt conditions (TE or equivalent low salt buffer or simply water) |
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No phenol/chloroform extraction |
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No organic or toxic reagents |
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No alcohol precipitation |
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A260/280 is always > 1.8 |
| Spin cups, columns and 96 well plates: The GENOMED silica membranes are supplied in micro-spin cups, 96 well plates or midi and maxi spin columns depending on the scale of preparation. |
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| Micro spin cup | Midi spin col | Maxi spin col | 96 well plate |
| The procedure to purify DNA always follows a simple and convenient "bind-wash-elute" procedure. DNA is bound to the silica membrane in the presence of high salt. Proteins, polysaccharides, dyes (if present) and other impurities are not adsorbed. After a wash step, pure DNA is eluted in a small volume of TE buffer or water. All GENOMED products containing silica membranes follow this simple and convenient procedure. | |||