GENOMED IEX-Resin

The GENOMED Anion Exchange Resin was developed in the early 90īs and became the core element of GENOMEDīs JETSTAR Plasmid Purification Kits in 1994. The JETSTAR kits are designed to achieve ultra pure plasmid and cosmid DNA from E.coli cells, which has biological activity equivalent to more than two rounds of CsCl gradient purification. Experiences over the last six years indicate that JETSTAR purified DNA is ideal for applications, like automated sequencing, transfection, microinjection and gene therapy research.

GENOMED IEX resin specifications:
	Solid-phase, anion exchange chromatography
	Porous silica based resin
	Silica beads with uniform particle size and very small pores of 60Å
	Extraordinary large surface area, because of the very small pores
	Ultra high charge density, because of the large surface
	Surface coated with positively charged DEAE groups
	DEAE groups are linked to resin surface by very long spacers
	Long spacers and the ultra high charge density provide an excellent separation range
	Ultra high charge density provides very high DNA capacity
	GENOMED IEX resin is applied in gravity fast flow columns
	Gravity fast flow columns are available in Mini, Midi and Maxi format
	Vacuum cartridges are available in Mega and Giga format
	Plasmid DNA of all sizes can be prepared
	Plasmid DNA recovery is 90% - 95% on average
	A260/280 ratio is > 1.85
	Endotoxin level is < 0.01 EU/ĩg plasmid DNA

Elution profil of the GENOMED IEX Resin

The GENOMED IEX Resin is the core element of all JETSTAR plasmid purification kits. The resin is applied in gravity fast flow columns of Mini, Midi and Maxi format. Column sizes indicate the amount of plasmid DNA to be isolated.
Mini column	Midi column	Maxi column
Mega cartridge	Giga cartridge	LFU-Unit
The procedure employs a modified alkaline/SDS method to prepare the cleared lysate. After neutralization, the lysate is applied onto a JETSTAR column or vacuum cartridge, and the plasmid DNA is bound to the anion exchange resin. One to two washes of the resin remove RNAs, proteins and all other impurities completely. Finally, the purified plasmid DNA is eluted in high salt buffer from the column and concentrated by an alcohol precipitation.

Associated GENOMED products:
	NoEndo JETSTAR ENDOTOXIN-FREE Plasmid MAXI Kit
	NoEndo JETSTAR ENDOTOXIN-FREE Plasmid MEGA Kit
	NoEndo JETSTAR ENDOTOXIN-FREE Plasmid MEGA FILTER Kit
	NoEndo JETSTAR ENDOTOXIN-FREE Plasmid GIGA Kit
	NoEndo JETSTAR ENDOTOXIN-FREE Plasmid GIGA FILTER Kit
	JETSTAR Plasmid Purification Mini Kit
	JETSTAR Plasmid Purification Midi Kit
	JETSTAR Plasmid Purification Maxi Kit
	JETSTAR / LFU Plasmid Purification Midi Kit
	JETSTAR / LFU Plasmid Purification Maxi Kit
	JETSTAR Plasmid Purification Mega Kit
	JETSTAR Plasmid Purification Giga Kit