DNA from Blood

JETQUICK Blood & Cell DNA Purification Kits (Mini, Midi, Maxi): The kits purify genomic DNA from whole blood, buffy coat, other body fluids and cell culture samples. Cells are lysed by a combination of a proteolytic enzyme, detergent and chaotropic salt. The lysate is directly applied onto the JETQUICK spin columns. The DNA is selectively bound to the highly specified silica membrane. Two subsequent washes remove residual contaminants, like proteins, metabolites, dyes, detergent etc. The purified DNA is eluted in TE buffer or water. The procedure is completed in 30 min. The DNA is ready-to-use.

JETFLEX Genomic DNA Purification Kit: The kit offers a quick and very simple solution for the purification of genomic DNA from a variety of sources, including whole blood, buffy coat, body fluids and cell culture samples. The DNA extraction is performed solely by aqueous solutions. Cell lysis is carried out by a detergent and can further be improved with proteinase K. After lysis proteins are selectively removed by a specially designed precipitation buffer. Finally, the genomic DNA is precipitated from the supernatant with isopropanol, washed and dried. The dry DNA pellet is redissolved in TE buffer or water. The procedure is completed in 20-25 min. The DNA is ready-to-use.