JETQUICK Tissue
JETFLEX Genomic
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| Genomed has developed two different kits to extract
and purify genomic DNA from solid tissue samples. With both kits
the DNA is set free by an enzymatic digest of the material with
proteinase K and detergent. In the following steps of the JETQUICK
Tissue DNA Purification Kit a spin cup procedure is used, whereas
the JETFLEX Tissue DNA Purification Kit follows a procedure without
any binding matrix. |
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JETQUICK Tissue DNA Purification Kit:
The kit purifies genomic DNA from solid tissue samples, like liver,
brain, kidney etc. The DNA in the solid tissue samples is set
free by an enzymatic digestion of the material with proteinase
K. The lysate is then mixed with detergent and chaotropic salt.
The mixture is directly applied onto a JETQUICK micro spin column.
The chaotropic salt facilitates selective binding of the DNA to
the highly specified silica membrane. Two subsequent washes remove
residual contaminants (proteins, metabolites, dyes etc.). The
purified DNA is eluted in TE buffer or water |
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JETFLEX Genomic DNA Purification Kit:
The kit offers a quick and very simple solution for the purification
of genomic DNA from solid tissue samples. The DNA extraction is
performed solely by aqueous solutions. Cells are lysed by a combination
of proteinase K and detergent. Then proteins are selectively removed
by a specially designed precipitation buffer.
Finally, the genomic DNA is precipitated from the supernatant
with isopropanol, washed and dried. The dry DNA pellet is redissolved
in TE buffer or water. |
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