JETWELL96 PCR

JETWELL96 PCR Product Purification Kit
	96 well plate

Advantages in comparison to competitor's products
	Very easy to use
	Very high speed of the procedure
	Strongly minimized risk of cross-contamination
	Short processing times because of very efficient flowthrough of sample, wash and elution buffer
	No blocking of wells
	No covering of unused wells required during centrifugation
	Even elution volumes
Specifications:
	Surface modified silica membrane
	High speed 96 well plate
	DNA capacity is 20 µg DNA per well
	Centrifugation procedure
	Purification of dsDNA fragments from PCR assays
	No mineral oil removal necessary
	No ethanol or isopropanol precipitation
	No phenol/chloroform extraction
	No organic or toxic reagents
	96 PCR assays can be purified simultaneously
	DNA fragments of 80-20.000 bp can be purified
	DNA recovery is 85-95 % on average
	Separates 80 bp DNA fragments from 40 mer primers
	Primer removal is > 99,8 %
	Total removal of nucleotides and polymerase
	Desalting and concentrating DNA are included
	Total procedure is 20-25 min per 96 preparations
	DNA elution in TE or equivalent low-salt buffer or simply water
	DNA is ready-to-use
Applications:
	Fluorescent sequencing
	Radioactive sequencing
	Ligation
	Restriction analysis
	Labeling
	In vitro transcription
	All other enzymatic reactions

A PCR product (121 bp) was contaminated by adding an excessive amount of a 40 nt long single-stranded oligo primer. Thereafter, the contaminated PCR products were purified using the JETWELL96 kit. From the left: A 100 bp ladder (lane 1 and 11), contaminated PCR product (lane 2), and purified PCR products (8 replicates) in lane 3 - 10.

Same experiment as above, but 516 bp PCR product was used.

Same experiment as above, but 1387 bp PCR product was used. (The bands of the PCR products in lanes 2 show a different behaviour, which is due to the very large amount of contaminating oligonucleotides).

Protocols: - JETWELL-96 PCR Purification Protocol     MSDS   - Buffer H1 - Buffer H2 - TE Buffer - JETWELL 96-well plate