Probe Purification

JETNICK Probe Purification Kit

Specifications:
	Product removes unincorporated 32P- and 35S-nucleotides from labeling assays
	Product contents are JETNICK resin, ready-to-use buffers and protocol
	DNA yield is 5µg DNA/20µl JETNICK resin
	Batch procedure with bulk resin following the "bind-wash-elute" principle
	No ethanol or isopropanol precipitation
	No phenol/chloroform extraction
	No organic or toxic reagents
	Desalting and concentrating DNA are included
	Removal of nucleotides is > 99.99%
	Removal of protein and salt is > 99.99%
	DNA sizes are 80bp - 20.000bp
	DNA recovery is up to 95% (even ng DNA amounts)
	DNA elution in 20µl TE or equivalent low-salt buffer or simply water
Applications:
	Radioactive sequencing
	Hybridization

The 448 bp DNA fragment of Bluescript II KS (PvuII digest) was 3´-labeled with 32P-dATP using Terminal Transferase. The removal of unicorporated nucleotides was carried out with ethanol precipitation according to Sambrook et al. 1989 (first lane), standard Sephadex column (second lane), spin column (third lane) and JETNICK (fourth lane), demonstrated by a 15% PAGE. The last lane shows the initial labeling assay.
Protocols: - JETNICK Probe Purification Protocol     Troubleshooting     Various: - JETNICK Extended Troubleshooting Guide     MSDS   - JETNICK MSDS