| JETQUICK Blood DNA Spin MINI Kit | |
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| Micro spin cup | |
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| JETQUICK Blood DNA Spin MINI Kit | |
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| Micro spin cup | |
| Specifications: | |
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Product is designed to prepare DNA from blood, buffy coat, cell cultures, body fluids etc. |
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Product contents are micro-spin cups, GENOMED protease, RNase A, ready-to-use buffers, protocol |
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For whole blood volumes of 200 µl - 1 ml |
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Membrane capacity is 50µg DNA per micro spin cup |
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Ultra quick procedure |
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No ethanol or isopropanol precipitation |
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No phenol/chloroform extraction |
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No organic or toxic reagents |
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Excellent reproducibility |
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Total procedure is 30min |
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DNA sizes are up to 50.000bp |
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DNA recovery is 85% - 95% on average |
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A260/280 ratio is > 1.8 |
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DNA elution in TE or equivalent low-salt buffer or simply water |
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Micro spin cups are adaptable to vacuum manifold |
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RNase included in the kit for RNA-free DNA |
| Applications: | |
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Amplification (PCR) |
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Blotting & Hybridization |
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Restriction digest |
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Viral diagnostics |
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Bacterial diagnostics |
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Fungal diagnostics |
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HLA typing |
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Cancer research |
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Human genetic testing |
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Paternity testing |
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Forensic analysis |
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| Genomic DNA was prepared from various blood samples using the JETQUICK Blood DNA Spin Kit. Aliquots were run on a 1% TAE agarose gel. First and last lane is the length standard Lambda HindIII/EcoRI. From left to right: EDTA-blood/fresh (lane 2), EDTA-blood/stored for 2 days at 4°C (lane 3), EDTA-blood/frozen (lane 4), Citrate-blood/fresh (lane 5), Citrate-blood/stored for 2 days at 4°C (lane 6), Citrate blood/frozen (lane 7), Heparin-blood/fresh (lane 8) and Heparin-blood/frozen (lane 9). | Genomic DNA was prepared from blood to amplify a 1387bp fragment of the DMD-Gene (Duchenne Muscular Dystrophy). Increasing amounts of DNA template were used; 5ng (lane 2), 10ng (lane3), 25ng (lane 4), 50ng (lane 5), 75 ng (lane 6), 100ng (lane 7), 150ng (lane 8) and 200ng (lane9). Lane 10 is empty. Lane 11-18 show the results of amplification under the same conditions as lane 2-9 when a competitor product to prepare genomic DNA was used. Lane 1 is the length standard lambda HindIII/EcoRI. | |
