PCR Product Purification

The polymerase chain reaction (PCR) has rapidly established itself as one of the most widely used techniques in molecular biology. It is commonly used in processes such as sequencing, cloning, probe preparation, clone screening, mapping etc. In many cases a post-purification of the DNA fragment to get rid of primers, nucleotides, polymerases and salts is required.

JETPURE PCR Product Purification Kit: The dsDNA (PCR product) is extracted from the PCR assay in a convenient batch procedure. The binding matrix is a surface-modified silica resin. The DNA is eluted from the resin in TE or equivalent low-salt buffer or simply water. This product also fully removes dye-labeled oligos and nucleotides (e.g. with Cy3- or Cy5-label) from sequencing and/or labeling mixes.

JETQUICK PCR Product Purification Spin Kit: The dsDNA (PCR product) is extracted from the PCR assay using micro spin cups. The binding matrix is a surface modified silica membrane. The DNA is eluted from the membrane in TE or equivalent low-salt buffer or simply water.

JETWELL96 PCR Product Purification Kit: Double-stranded DNA fragments from up to 96 PCR assays can be purified simultaneously using the JETWELL96 system. The PCR assays are mixed with a chaotropic reagent and are directly applied into the wells of the JETWELL plate. The dsDNA is selectively bound to the JETWELL membrane and the majority of impurities will flow through. One wash is usually sufficient to remove residual impurities (primers, salts, dNTPīs and polymerases). The purified DNA is eluted in TE or equivalent low-salt buffer or simply water. The procedure is completed in 20-25 min. The DNA is ready-to-use. All steps of the procedure are carried out by centrifugation.