| Specifications: |
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Product is designed to extract DNA fragments from all types of agarose gels |
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Product contents are JETSORB resin, ready-to-use buffers and protocol |
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Resin capacity is 10µg DNA/10µl JETSORB resin |
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Batch procedure with bulk resin following the "bind-wash-elute" principle |
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No ethanol or isopropanol precipitation |
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No phenol/chloroform extraction |
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No organic or toxic reagents |
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Excellent reproducibility |
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DNA extraction from High Melting Point and Low Melting Point agarose gels |
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DNA extraction from TAE, TBE and TPE agarose gels |
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Gel slices with up to 2% agarose concentration can be used |
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No shearing of large DNA fragments |
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Total procedure is 25min |
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DNA sizes are 20bp - 45.000bp |
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DNA recovery is 85% - 95% on average |
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DNA elution in TE or equivalent low-salt buffer or simply water |
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| Applications: |
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Ligation |
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Labeling |
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Library screening |
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PCR template preparation |
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Radioactive sequencing |
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In-vitro transcription |
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All other enzymatic reactions |