JETQUICK Gel

JETQUICK Gel Extraction Spin Kit
	Micro-spin cup

Specifications:
	Product is designed to extract DNA fragments from all types of agarose gels
	Product contents are JETQUICK micro spin cups, ready-to-use buffers and protocol
	Resin capacity is up to 20µg DNA per micro spin cup
	Ultra quick procedure
	No ethanol or isopropanol precipitation
	No phenol/chloroform extraction
	No organic or toxic reagents
	Excellent reproducibility
	DNA extraction from High Melting Point and Low Melting Point agarose gels
	DNA extraction from TAE, TBE and TPE agarose gels
	Gel slices with up to 2% agarose concentration can be used
	No shearing of large DNA fragments
	Total procedure is < 25min
	DNA sizes are 40bp - 10.000bp
	DNA recovery is 85% - 95% on average
	DNA elution in TE buffer or water
	Micro-spin cups are adaptable to vacuum manifold (see protocol links below)
Applications:
	Ligation
	Labeling
	Library screening
	PCR template preparation
	Radioactive sequencing
	Fluorescent sequencing
	In-vitro transcription
	All other enzymatic reactions

The Invitrogen 1kb ladder was run on a 1% TAE agarose gel. Single bands of this length standard were extracted using the JETQUICK Gel Extraction Spin Kit. The purified fragments were run again on a 1% TAE agarose gel.		EcoRI digested pBluescript II KS (2961bp) was extracted from a 1% TAE agarose gel using the JETQUICK Gel Extraction Spin Kit. Ligations were set up for 2 hours at 25°C with 0.5 to 3.0 units of T4 DNA ligase in 0.5U increments (lane 3-8). Lane 2 is the unligated plasmid DNA.
Why using the JETQUICK ‚Gel Extraction Kit' and not a competitor's product?     Protocols: - JETQUICK Plasmid/PCR/Gel Extraction Protocol - JETQUICK Gel Extraction Protocol (Vacuum Option)     Troubleshooting   - JETQUICK Plasmid/PCR/Gel Extraction Extended Troubleshooting Guide     MSDS   - Buffer L1 - Buffer L2 - TE Buffer - JETQUICK Micro-Spin Units