NoEndo JETSTAR ENDOTOXIN-FREE 
 
  Endotoxins
 
 
NoEndo JETSTAR ENDOTOXIN-FREE Plasmid Purification Kit
 
  NoEndo Maxi column NoEndo Giga cartridge
 
 
Advantages:
 
Endotoxin content of purified DNA <0.001 EU/µg
TWO endotoxin removal steps instead of only 1
VERY QUICK processing times of vacuum-driven MEGA and GIGA cartridges
No additional incubation times in comparison to the JETSTAR standard protocol
Bacterial lysate remains CLEAR during endotoxin removal procedure
Short, convenient protocol
All components included in the kit
Buffers crucial for endotoxin removal tested and certified to contain <0.1 endotoxin units (EU) per ml
 

 

NoEndo JETSTAR specifications:
 
Product is designed to prepare ENDOTOXIN-FREE plasmid DNA from E. coli cells (maxi, mega and giga scale)
Endotoxin level of purified plasmid DNA is <0.001 EU/µg plasmid DNA
Product contents are gravity fast flow columns or ultra-quick vacuum cartridges, ready-to-use endotoxin removal and chromatography buffers, RNase A and protocol
No chromosomal DNA contamination
No phenol/chloroform extractions
No organic or toxic reagents
Excellent reproducibility
Total column procedure in 10 min (MEGA) – 45 min (Maxi)
Plasmid DNA is ultrapure (better than 2 x CsCl) with no known application limit
Plasmid DNA recovery is 90 – 95% on average
Plasmid DNA of all sizes can be prepared
Plasmid DNA yields are up to 750 µg (Maxi) / 2,500 µg (Mega) / 10,000 µg (Giga)
A260/280 ratio is > 1.85
 

 

Applications for plasmid and cosmid DNA
 
Transfection of primary cells (incl. neuronal cells)
Transfection of very endotoxin-sensitive eucaryotic cells
Microinjection into animals and eucaryotic cells
Gene therapy applications
Capillary sequencing
All fluorescent sequencing protocols
Manual sequencing
All standard cloning and enzymatic manipulations
 

Sequencing result for JETSTAR-purified plasmid DNA
 
Why using the NoEndo JETSTAR ENDOTOXIN-FREE Plasmid Purification System and not a competitor´s product?
  MAXI
  MEGA
  GIGA
   
Protocols: NoEndo JETSTAR 2.0 ENDOTOXIN-FREE
- Plasmid MAXI Protocol
- Plasmid MEGA/GIGA Protocol
- Plasmid MEGA/GIGA FILTER Protocol
   
Troubleshooting info:
 
For low or no DNA yield: - Diagnosis Protocol
Various: - Extended Troubleshooting Guide
   
Material Safety Data Sheets (MSDS)
  - Buffer E1
- Buffer E2
- Buffer E3
- Buffer ENDO-1
- Buffer E4
- Buffer ENDO-2
- Endotoxin-free Buffer E5
- Endotoxin-free Buffer E6
- RNase
- Endotoxin-free TE Buffer
- JETSTAR Maxi Columns
- JETSTAR Mega/Giga Cartridges
- JETFILTER