Genomed

Advantages:

	Endotoxin content of purified DNA <0.001 EU/µg
	TWO endotoxin removal steps instead of only 1
	VERY QUICK processing times of vacuum-driven MEGA and GIGA cartridges
	No additional incubation times in comparison to the JETSTAR standard protocol
	Bacterial lysate remains CLEAR during endotoxin removal procedure
	Short, convenient protocol
	All components included in the kit
	Buffers crucial for endotoxin removal tested and certified to contain <0.1 endotoxin units (EU) per ml

NoEndo JETSTAR specifications:

	Product is designed to prepare ENDOTOXIN-FREE plasmid DNA from E. coli cells (maxi, mega and giga scale)
	Endotoxin level of purified plasmid DNA is <0.001 EU/µg plasmid DNA
	Product contents are gravity fast flow columns or ultra-quick vacuum cartridges, ready-to-use endotoxin removal and chromatography buffers, RNase A and protocol
	No chromosomal DNA contamination
	No phenol/chloroform extractions
	No organic or toxic reagents
	Excellent reproducibility
	Total column procedure in 10 min (MEGA) – 45 min (Maxi)
	Plasmid DNA is ultrapure (better than 2 x CsCl) with no known application limit
	Plasmid DNA recovery is 90 – 95% on average
	Plasmid DNA of all sizes can be prepared
	Plasmid DNA yields are up to 750 µg (Maxi) / 2,500 µg (Mega) / 10,000 µg (Giga)
	A260/280 ratio is > 1.85

Applications for plasmid and cosmid DNA

	Transfection of primary cells (incl. neuronal cells)
	Transfection of very endotoxin-sensitive eucaryotic cells
	Microinjection into animals and eucaryotic cells
	Gene therapy applications
	Capillary sequencing
	All fluorescent sequencing protocols
	Manual sequencing
	All standard cloning and enzymatic manipulations

Endotoxins


NoEndo JETSTAR ENDOTOXIN-FREE Plasmid Purification Kit


	NoEndo Maxi column	NoEndo Giga cartridge