JETSTAR/LFU 
 
  Endotoxins
 
 
JETSTAR/LFU Plasmid Purification Kit
 
  LFU-Unit Midi column Maxi column
 
 
Advantages:
 
Very convenient procedure - much simpler and shorter than any competitor's protocol
Great time-saving compared to standard procedure
Newly designed FAST Midi columns much faster than any comparable product
Larger culture volumes can be processed on the new FAST Midi columns
No centrifugation necessary to clear bacterial lysate
Lysate clarification integrated into column procedure
Only ONE column wash for the new FAST Midi and Maxi columns
No clogging of the lysate filter unit
Much less susceptible against overloading than competitor's products
 

 

JETSTAR specifications:
 
Product is designed to prepare plasmid DNA (medium and large scale) from E.coli cells
Product contents are gravity fast flow columns, RNase A, ready-to-use buffers and protocol
JETSTAR / LFU preparations are available in Midi and Maxi format
JETSTAR is able to prepare cosmid DNA and BAC DNA as well
No chromosomal DNA contamination
No phenol/chloroform extraction
No organic or toxic reagents
Excellent reproducibility
Total procedure in 30 - 40 min
Plasmid DNA is ultrapure (better than 2 x CsCl)
Plasmid DNA recovery is 90% - 95% on average
Plasmid DNA of all sizes can be prepared
Plasmid DNA yields are up to 200 µg (Midi) and up to 850 µg (Maxi)
A260/280 ratio is > 1.85
Endotoxin level is < 0.01 EU/µg plasmid DNA
 

 

Applications for plasmid and cosmid DNA
 
Transfection
Manual and automated sequencing, including capillary sequencing
Microinjection
In vitro transcription
Restriction analysis, ligation, labeling etc.
Transfection of endotoxin-sensitive cells (Endo Free)
Gene therapy applications
 

Sequencing result for JETSTAR-purified plasmid DNA
 
Why using JETSTAR 2.0/LFU and not a competitor´s product?
   
Protocols:
- JETSTAR 2.0/LFU Plasmid Midiprep Protocol
- JETSTAR 2.0/LFU Plasmid Maxiprep Protocol
   
Troubleshooting info:
 
For low or no DNA yield: - Diagnosis Protocol
Various: - Extended Troubleshooting Guide
   
Material Safety Data Sheets (MSDS)
  - Buffer E1
- Buffer E2
- Buffer E3
- Buffer E4
- Buffer E5
- Buffer E6
- RNase
- TE Buffer
- Star - Columns
- Mega-/Giga-Cartridges
- JETFILTER