JETPREP

JETPREP Plasmid Miniprep Kit

Specifications:
	Product is designed to prepare plasmid DNA (small scale) from E.coli cells
	Product contents are JETPREP resin, RNAse A, ready-to-use buffers and protocol
	Plasmid yield is up to 15µg DNA
	Batch procedure with bulk resin following the "bind-wash-elute" principle
	No ethanol or isopropanol precipitation
	No phenol/chloroform extraction
	No organic or toxic reagents
	Excellent reproducibility
	Culture volumes up to 3ml
	Plasmid sizes up to 15.000bp
	Plasmid recovery is 80% - 90% on average
	A260/280 ratio is approx. 1.8
	Plasmid DNA elution in 30 µl - 90 µl TE or equivalent low-salt buffer or simply water
Applications:
	Ligation
	Labeling
	Library screening
	PCR template preparation
	Radioactive sequencing
	All other enzymatic reactions

A number of eight plasmid preparations were carried out simultaneously from eight separate 1 ml E.coli cultures using the JETPREP Plasmid Miniprep Kit. The plasmid was pT7T3 19U. Aliquots were run on a 1% agarose gel.
Protocols: - JETPREP Plasmid Mini - Protocol     Troubleshooting   - JETPREP Extended Troubleshooting Guide     MSDS   - Buffer D1 - Buffer D2 - Buffer D3 - Buffer D4 (incl. JETPREP Resin) - Buffer D5 - RNase A