| JETSPIN Plasmid Purification Kit | ||
   |
||
| Maxi spin cup | Midi spin cup | |
|
||
| JETSPIN Plasmid Purification Kit | ||
|
|
||
| Maxi spin cup | Midi spin cup | |
| Specifications: | |
 |
Product is designed to prepare plasmid DNA (medium and large scale) from E.coli cells |
|
Recommended for high copy plasmid DNA |
|
Membrane capacity is 100µg DNA (Midi format) and 500µg DNA (Maxi format) in minimum |
|
Product contents are JETSPIN columns, RNAse A, ready-to-use buffers and protocol |
|
High speed spin column procedure |
|
No ethanol or isopropanol precipitation |
|
No phenol/chloroform extraction |
|
No organic or toxic reagents |
|
Excellent reproducibility |
|
Culture volumes up to 50 ml (Midi) and up to 100 ml (Maxi) |
|
Total procedure is 25min |
|
Plasmid sizes up to 20.000bp |
|
Plasmid recovery is 85% - 90% on average |
|
A260/280 ratio is > 1.8 |
|
DNA elution in TE or equivalent low-salt buffer or simply water |
|
Plasmid can be used for all in vitro-applications (see below) |
| Applications: | |
|
Fluorescent sequencing |
|
Radioactive sequencing |
|
Ligation |
|
Labeling |
|
PCR template preparation |
|
All other enzymatic reactions |
 |
 |
|||||||||||||||||
| Recombinant plasmid DNA (pT7T3 19U, 1.7kb insert) was prepared using the JETSPIN Plasmid Kit. The purified DNA was digested with various restriction enzymes, such as BamHI (lane 2), BglI (lane 3), DdeI (lane 4), DraI (lane 5), EcoRI (lane 6), HaeIII (lane 7), HindIII (lane 8), HpaII (lane 9), PvuI (lane 10), PvuII (lane 11), PvuI/PvuII (lane 12), RsaI (lane 13) and BglI/NdeI (lane 14). Lane 1 shows the undigested plasmid DNA. | Plasmid DNA (pT7T3 19U) was prepared using the JETSPIN Plasmid Kit. The plasmid DNA was digested with EcoRI and ligated with a 1.7kb DNA fragment (lane 2 shows the DNAs before ligation). Ligations were set up for 1h at 25°C with 0.03 U (lane 3), 0.06 U (lane 4), 0.08 U (lane 5), 0.1 U (lane 6) and 0.12 U (lane 7) of T4 DNA ligase per µg plasmid DNA. Length standard in lane 1 is the BRL 1kb ladder. | |||||||||||||||||
| Midi and Maxi preparations: The JETSPIN Plasmid Kits are offered with Midi and Maxi JETSPIN Columns for large-scale plasmid preparations. No vacuum manifold, special racks or any other additional auxiliaries are required. The spin columns can be used with any standard bench or floor model centrifuge with an appropriate rotor that can hold 50 ml centrifuge tubes (i.e. Falcon, Greiner, Sarstedt). A swinging-bucket type rotor is an absolute requirement. Centrifugations are carried out at low g-forces (5000 x g). Regardless of which JETSPIN Column format is used, the time of plasmid preparation is no more than 25 min. |
||||||||||||||||||
|
||||||||||||||||||