JETQUICK 
 
JETQUICK Plasmid Purification Spin Kit
 
  Micro spin cup
 
Specifications:
 
Product is designed to prepare plasmid DNA (small scale) from E.coli cells
Product contents are micro spin cups, RNAse A, ready-to-use buffers and protocol
Very high DNA capacity (up to 50 µg plasmid DNA per micro-spin cup)
Ultra quick procedure using micro spin cups
No chromosomal DNA contamination
No ethanol or isopropanol precipitation
No phenol/chloroform extraction
No organic or toxic reagents
Excellent reproducibility
Culture volumes up to 5ml
Total procedure is < 25min
Plasmid sizes are up to 20.000bp
Plasmid recovery is 90% - 95% on average
Plasmid quality matches CsCl purified DNA
A260/280 ratio is > 1.8
DNA elution in TE buffer or equivalent low-salt buffer or simply water
Micro-spin cups are adaptable to vacuum manifold (see protocol links below)
 
Applications:
 
Fluorescent sequencing
Ligation
Restriction analysis
PCR template preparation
Labeling
All other enzymatic reactions
 
 
   
 
A number of twelve plasmid preparations were carried out simultaneously from twelve separate 1 ml E.coli cultures using the JETQUICK Plasmid Miniprep Kit. The plasmid was pT7T3 19U. Aliquots were run on a 1% agarose gel.    
     
Why using JETQUICK ‚Plasmid' and not a competitor's product?
   
Protocols:
- JETQUICK Plasmid/PCR/Gel Extraction Protocol
- JETQUICK Plasmid Miniprep Protocol (Vacuum Option)
   
Troubleshooting
  - JETQUICK Plasmid/PCR/Gel Extraction Extended Troubleshooting Guide
   
MSDS
  - Buffer G1
- Buffer G2
- Buffer G3
- Buffer GX
- Buffer G4
- TE Buffer
- RNase A
- JETQUICK Micro-Spin Units