| JETSTAR specifications: |
| |
 |
Product is designed to prepare plasmid DNA (small, medium and large scale)
from E.coli cells |
|
Product contents are gravity fast flow columns or vacuum cartridges, RNAse A, ready-to-use buffers and protocol |
|
JETSTAR preparations are available in Mini, Midi, Maxi, Mega and Giga format |
|
JETSTAR is able to prepare cosmid DNA and BAC DNA as well |
|
No chromosomal DNA contamination |
|
No phenol/chloroform extraction |
|
No organic or toxic reagents |
|
Excellent reproducibility |
|
Total procedure is 40 - 60min |
|
Plasmid DNA is ultrapure (better than 2 x CsCl) |
|
Plasmid DNA recovery is 90% - 95% on average |
|
Plasmid DNA of all sizes can be prepared |
|
Plasmid DNA yields are 20µg (Mini), 100µg (Midi), 500µg (Maxi), 2.500µg (Mega), 10.000µg (Giga) in minimum |
|
A260/280 ratio is > 1.85 |
|
Endotoxin level is < 0.01 EU/µg plasmid DNA |
| |
| Applications for plasmid and cosmid DNA |
| |
|
Transfection |
|
Manual and automated sequencing, including capillary sequencing |
|
Microinjection |
|
In vitro transcription |
|
Restriction analysis, ligation, labeling etc. |
|
Transfection of endotoxin-sensitive cells (Endo Free) |
|
Gene therapy applications |
