DNA from Blood (Midi/Maxi) 
 
JETQUICK Blood DNA Midi/Maxi Kit
 
  Midi spin cup Maxi spin cup
 
Specifications:
 
Product is designed to prepare DNA from blood, buffy coat, cell cultures, body fluids etc.
Product contents are midi or  maxi spin cups, GENOMED protease, RNase A, ready-to-use buffers, protocol
MIDI: For whole blood volumes of 1 - 5 ml
MAXI: For whole blood volumes of 5 - 20 ml
Membrane capacity is up to 150 µg per Midi-Spin Cup
Membrane capacity is up to 600 µg per Maxi-Spin Cup
Ultra quick procedure
No ethanol or isopropanol precipitation
No phenol/chloroform extraction
No organic or toxic reagents
Excellent reproducibility
Total procedure is 30min
DNA sizes are up to 50.000bp
DNA recovery is 85% - 95% on average
A260/280 ratio is > 1.8
DNA elution in TE or equivalent low-salt buffer or simply water
Midi and Maxi spin cups are adaptable to vacuum manifold
RNase included in the kit for RNA-free DNA
 
Applications:
 
Amplification (PCR)
Blotting & Hybridization
Restriction digest
Viral diagnostics
Bacterial diagnostics
Fungal diagnostics
HLA typing
Cancer research
Human genetic testing
Paternity testing
Forensic analysis
 
 
 
 
Gel analysis of DNA from 24 blood samples prepared in parallel with the JETQUICK Blood DNA Midi kit. 500 ng blood DNA per lane were applied onto a 0.8% TAE agarose gel (lanes 2 - 25). Lambda DNA digested with EcoRI / Hind III was used as DNA size marker in lanes 1 and 26.   Gel analysis of DNA from 6 blood samples prepared in parallel with the JETQUICK Blood DNA Maxi kit. 700 ng blood DNA per lane were applied onto a 0.8% TAE agarose gel (lanes 2 - 7). Lambda DNA digested with EcoRI / Hind III was used as DNA size marker in lanes 1 and 8.
     
Why using JETQUICK ‚Blood & Cell Culture' and not a competitor's product?
   

Protocols:
- JETQUICK Blood Midi Protocol (for 1 - 5 ml whole blood)
- JETQUICK Blood Maxi Protocol (for 5 - 10 ml whole blood)
- JETQUICK Blood Special Maxi Protocol (up to 20 ml whole blood)

 

- JETQUICK Cell Culture MIDI Protocol (up to 1 x 108 cells/assay)
- JETQUICK Cell Culture MAXI Protocol (up to 5 x 108 cells/assay)

   
Troubleshooting
   
Various: - JETQUICK `Blood & Cell Culture`Extended Troubleshooting
  Guide
   
MSDS
  - Buffer K1
- Buffer K2
- Buffer KX
- TE Buffer
- GENOMED Protease
- RNase A
- JETQUICK Micro-Spin Units