| JETFLEX Genomic DNA Kit | |
 |
|
|
||
| JETFLEX Genomic DNA Kit | |
|
|
| Specifications: | |
 |
High speed buffer procedure |
|
DNA Purification from whole blood, buffy coat etc. |
|
DNA yield is 5-10 µg per 300 µl whole blood |
|
No phenol/chloroform extraction |
|
No organic or toxic reagents |
|
Preparation size: can be downscaled or upscaled |
|
DNA sizes up to 150.000 bp can be purified |
|
A260/280 ratio is > 1.8 |
|
Total procedure is 25-30 min |
|
DNA dissolution in TE or equivalent low-salt buffer or simply water |
|
DNA is ready-to-use |
|
Proteinase K included for hard-to-lyse samples |
|
RNase included for RNA-free DNA |
| Applications: | |
|
Amplification |
|
Blotting |
|
Restriction analysis |
|
Viral diagnostics |
|
Bacterial diagnostics |
|
Fungal diagnostics |
|
HLA typing |
|
Cancer research |
|
Human genetic testing |
|
Paternity testing |
|
Forensic analysis |
 |
||
| Genomic DNAs from above lanes 2 - 13 were used as a templates for PCR. The amplification product is a 3.5 kb fragment (lanes 2 -13). 50 ng of DNA were used as template in each assay. Lanes 1 and 14 are size markers as described above. |
 |
||
| Genomic DNAs from above lanes 2 - 13 were used as a templates for PCR. The amplification product is a 500 bp fragment (lanes 2 -13). 50 ng of DNA were used as template in each assay. Lanes 1 and 14 are size markers as described above. | ||
