DNA Size Markers 
 
JETWAY DNA Size Markers
 
The JETWAY product line consists of a number of very useful DNA size markers for research use. All JETWAY DNA size markers are showing the following quality features:

• The plasmids being the origin of the DNA size markers are propagated in nuclease-depleted E. coli strains.

• Restriction enzymes used for the preparation of the DNA size markers are of the highest quality with nearly undetectable nuclease content. This ensures a maximum of functional 5‘ and/or 3‘-ends, that can be used for labeling.

• Nearly undetectable background due to the absence of nucleases. Excellent signal-to-noise ratio.

• All DNA size markers are deproteinized by a phenol/chloroform extraction and subsequently lyophilized, thus ensuring very high DNA stability even after dissolving of the size markers in loading buffer.

• DNA bands stable for at least 4 years at –20°C and 1-2 weeks at 37°C. No DNA degradation observed within this time frame.

• The deproteinisation of the DNA size markers ensures a very precise and reproducible gel pattern even on different gels. Different running behaviour due to still present proteins that were not removed will be prevented.

• All JETWAY DNA size markers contain only DNA bands with sizes, that are relevant in the lab. DNA bands with extraordinary high molecular weights, that are not useful for most experiments, but nonetheless present in many other commercialy available DNA markers, are avoided in the JETWAY product line.
 

Link:

- Product insert 2176
 
JETWAY 2176:
 
Type of marker:
Number of fragments:
Fragment sizes (in bp): 		mixed pBR 328/Hinf I and pBR 328/Bgl I fragments
12
2176, 1766, 1230, 1033, 653, 517, 453, 394, 298, 234,
220, 154
Application range: -Gel analysis of routine cloning experiments,
-Gel analysis of mid-size PCR fragments (250 bp – 2 kb),
 amplified from e.g. high- molecular weight DNA
 
 
Link::

- Product insert 501
 
JETWAY 501:
 
Type of marker:
Number of fragments:
Fragment sizes (in bp): 		pUC 19/Mspl fragments
13
501, 489, 404, 331, 242, 190, 147, 111, 110, 67, 34, 34, 26
Application range:  

-Gel analysis of small-size PCR fragments

 (50 bp – 500 bp), amplified from e.g. low- molecular

 weight DNA
-The JETWAY 501 DNA size marker shows a very
 characteristic gel pattern even after short run times and
 even before the single DNA bands have fully separated.
 Very suitable for those users who do not want to spend
 too much time on gel analysis.

 
 
Link:

- Product insert 1000
 
JETWAY 1000:
 
Type of marker:

Number of fragments:
Fragment sizes (in bp): 		100 bp ladder (equimolar); (EcoRI fragments of various plasmids)
11
1000, 900, 800, 700, 600, 500 (2x), 400, 300, 200, 150, 100
Application range:  

 

-Suitable for general analysis of DNA fragments and PCR

 products

-All fragments have EcoRI-ends, thus allowing very easy

 and uniform labeling

-JETWAY 1000 is an equimolar DNA size marker, which

 means, that the number of moles of all DNA bands is

 identical.

 This leads to a stronger appearance of the DNA bands

 with higher molecular weight on the gel. A longer gel run is

 necessary to fully separate all DNA bands.

-Typically 0.6 – 1.0 ΅g of this marker should be applied

 per lane on an analytical gel.

 
 
Link:

- Product insert 1011
 
JETWAY 1011:
 
Type of marker:

Number of fragments:
Fragment sizes (in bp): 		100 bp ladder (equalized); (EcoRI fragments of various plasmids)
11
1000, 900, 800, 700, 600, 500 (2x), 400, 300, 200, 150, 100
Application range:  

 

-Suitable for general analysis of DNA fragments and PCR

 products

-All fragments have EcoRI-ends, thus allowing a very easy

 labeling. As the smaller DNA bands are present with

 higher masses, their appearance when labeled will be

 much stronger.

-JETWAY 1011 is an equalized DNA size marker, which

 means, that all bands will appear on a gel with nearly

 identical intensity. To achieve this homegeneous

 appearance of all bands on the gel, the JETWAY 1011

 marker contains e.g. six times the amount of the 100 bp

 band compared to the JETWAY 1000 (equimolar) marker.

-Due to the homogeneous appearance of all bands only

 0.25 ΅g of the equalized JETWAY 1011 marker are to

 be used per lane. This is just 1/3 – 1/4 in comparison

 to the JETWAY 1000 (equimolar) marker.

 
 
Link::

- Product insert 1500
 
JETWAY 1500:
 
Type of marker:
Number of fragments:
Fragment sizes (in bp): 		EcoRI fragments of various plasmids
7
1500, 1200, 1100, 1000, 900, 800, 500
Application range:  

-Ideal DNA size marker for ‚high-throughput‘ gel analysis

-Ideally suited for the size determination of PCR

 fragments within a range of 500 bp – 1.500 bp

-Allows a quick estimate of the size of an unknown DNA

 fragment even after short run-times.

-Enriched, stronger appearing 1.000 bp band (140 ng

 when 0,5 ΅g DNA marker are loaded onto the gel) for

 appraisement of the mass of a band with unknown DNA

 amount.

 
 
Link::

- Product insert 5000
 
JETWAY 5000:
 
Type of marker:

Number of fragments:
Fragment sizes (in bp): 		100 bp ladder (extended); (EcoRI fragments of various plasmids)
17
5000, 4000, 3000, 2500, 2000, 1500, 1000, 900, 800, 700, 600, 500 (2x), 400, 300, 200, 150, 100
Application range:  

-Universal DNA size marker for plasmids and DNA

 fragments within a size range of 100 bp – 5 kb

-All fragments have EcoRI-ends, thus allowing very easy

 and uniform labeling

-Typically 0.8 – 1.0 ΅g of this marker should be applied

 per lane on an analytical gel.

 
 
Link::

- Product insert 8000
 
JETWAY 8000:
 
Type of marker:
Number of fragments:
Fragment sizes (in bp): 		250 bp ladder (EcoRI fragments of various plasmids)
16
8000, 6000, 5000, 4000, 3000, 2750, 2500, 2250, 2000, 1750, 1500, 1250, 1000, 750, 500, 250
Application range:   

-JETWAY 8000 DNA size marker recommended for

 size determination of plasmids and their insertions within

 a size range of 250 bp – 8 kb.

-Masses of DNA fragments from 250 – 1.000 bp

 enriched and from 3.000 – 8.000 bp reduced to ensure

 a homogeneous appearance of all bands on a gel.

-Only 0.7 ΅g of this marker are necessary per lane