DNA from Tissue

JETFLEX Genomic DNA Kit

Specifications:
	High speed buffer procedure
	DNA Purification from solid tissue samples
	DNA yield is 20-150 µg per 10 mg animal tissue
	No phenol/chloroform extraction
	No organic or toxic reagents
	Preparation size: can be downscaled or upscaled
	DNA sizes up to 150.000 bp can be purified
	A260/280 ratio is > 1.8
	Total procedure is 25-30 min
	DNA dissolution in TE or equivalent low-salt buffer or simply water
	DNA is ready-to-use
	Proteinase K included for hard-to-lyse samples
	RNase included for RNA-free DNA
Applications:
	Amplification
	Blotting
	Restriction analysis
	Viral diagnostics
	Bacterial diagnostics
	Fungal diagnostics
	HLA typing
	Cancer research
	Human genetic testing
	Paternity testing
	Forensic analysis

Gel analysis of DNA from 12 identical tissue samples purified with the JETFLEX Genomic DNA Purification Kit (prepared in parallel). 2µg DNA per lane were loaded onto a 0.8 % TAE agarose gel. Lambda-DNA (digested with HindIII and EcoRI) was used as DNA size marker in lanes 1 and 14 (from the left). The size of the prepared genomic DNA is > 50 kb, typically 100-200 kb.

- Why using JETFLEX and not a competitor's product?     Protocols: - JETFLEX Genomic DNA Isolation Protocol    (NEW with ‚Buccal Swab‘ Protocol, Sept. 2005)      Troubleshooting   - JETFLEX Extended Troubleshooting Guide     MSDS   - Buffer RBC - Buffer CLB - Buffer PPT - `Pellet Compactor` - DNA Hydration Buffer - Proteinase K - RNase A