| JETWELL96 DNA Clean Up Kit | |
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| 96 well plate | |
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| JETWELL96 DNA Clean Up Kit | |
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| 96 well plate | |
| Advantages in comparison to competitor's products: | |
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Very easy to use |
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Very high speed of the procedure |
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Strongly minimized risk of cross-contamination |
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Short processing times because of very efficient flowthrough of sample, wash and elution buffer |
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No blocking of wells |
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No covering of unused wells required during centrifugation |
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Even elution volumes |
| Specifications: | |
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Surface modified silica membrane |
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High speed 96 well plate |
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DNA capacity is 20 µg DNA per well |
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Centrifugation procedure |
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DNA Purification from all kind of solutions and assays |
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All types of DNA can be purified (linear, circular,...) |
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No ethanol or isopropanol precipitation |
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No phenol/chloroform extraction |
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No organic or toxic reagents |
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96 assays can be purified simultaneously |
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DNA sizes of up to 20.000 bp can be purified |
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DNA recovery is up to 95 % (even ng DNA amounts) |
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Salt removal is 100 % |
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Dye removal is 100 % |
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Enzyme removal is > 99.8 % |
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Total procedure is 20-25 min |
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DNA elution in TE or equivalent low-salt buffer or simply water |
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DNA is ready to use |
| Applications: | |
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Fluorescent sequencing |
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Radioactive sequencing |
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Ligation |
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Restriction analysis |
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Labeling |
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In vitro transcription |
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All other enzymatic reactions |
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| Plasmid DNA (plasmid pEE8) was restricted with various restriction enzymes and sub-sequently purified over a JETWELL96 General DNA CleanUp plate. Lambda DNA (digested with HindIII and EcoRI) was used as DNA size marker in lanes 1 and 11 (from the left). Lane 2 shows undigested plasmid DNA, lane 3 plasmid DNA digested with DraI, lane 4 with RsaI, lane 5 with NdeI, lane 6 with HindIII, lane 7 with PstI, lane 8 with EcoRI, lane 9 with PvuII and lane 10 with HpaII. Each restriction assay was carried out with 2 µg of plasmid DNA. |
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| Ligation assays of plasmid pEE8 purified over a JETWELL96 General DNA CleanUp plate after complete digestion. Restriction assays from above were used. Ligations were set up for 1h at 25oC with 0.1U of T4 DNA ligase per µg of DNA (lanes 3 -10). Lambda DNA (digested with HindIII and EcoRI) was used as DNA size marker in lanes 1 and 11. Lane 2 shows undigested plasmid DNA. | ||||||||||||||
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